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qPCR-centered quantitative microbiome profiling is relevant also for samples that contains a considerable degree of host or non-bacterial DNA, by which bacterial density cannot be reliably approximated by complete DNA generate [five].

qPCR is Expense-successful and obtainable since the laboratory settings, machinery and reagents are much like Individuals necessary for getting ready the NGS libraries.

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The usage of relative abundance from following generation sequencing (NGS) data can result in misinterpretations of microbial Group constructions as due to compositionality, the relative abundances with the taxa becoming mutually dependent. This means that an increase of one taxon inevitably results in the concurrent decrease of one other(s). Since the changes of elements are mutually dependent, substantial Fake discovery fees arise when compositional data are analyzed utilizing standard statistical procedures [1].

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